R&D센터


연구소소개

㈜바이오노트의 생명과학연구소는 2004년도에 한국과학기술진흥협회로부터 기업부설연구소로 인정받아, 신제품 개발, 신기술 개발, 제품 개선을 담당하고 있습니다.
연구소는 크게 항원 개발팀, 항체 개발팀, 배양 정제팀, 제제화팀, 분자 진단팀으로 구성되어져 있습니다.

소비자들의 요구에 맞는 신제품을 개발하기 위하여 언제나 노력하고 있으며, 제품의 보완점을 지속적으로 연구하여 키트의 안정성과 특이도, 민감도를 향상시키고 있습니다.

 

항원 개발팀

배양 정제팀

분자 진단팀

제제화팀

항체 개발팀

 

연구소 경쟁력


본사의 연구소는


세계 유일의 금입자 접합체 및 클론 생산 능력 보유하고 있어 금입자접합체(Gold Conjugate)를 세계 4번째로 상용화에 성공하였고,


유전자 재조합 항원 제조기술 보유하고 있으며 특히 당사 고유의 고발현 Vector System과 단백질 refolding 기술을 가지고 있으며,


제제화의 핵심기술인 미생물 대량 생산 기술, 세포 배양기술, 효소 접합체 제조 기술, 형광물질 접합체 제조 기술, 단클론 항체 생산 기술을 가지고 있습니다.


이러한, 기술력을 바탕으로 본사 키트는 세계 최초의 제품(조류인플루엔자 신속진단키트 및 ELISA키트, 부루셀라 신속진단 키트 등)들을 개발할 수 있었습니다.

 

발표논문_Publication

1. Song D, Moon HJ, An DJ, Jeoung HY, Kim H, Yeom MJ, Hong M, Nam JH, Park SJ, Park BK, Oh JS, Song M, Webster RG, Kim JK, Kang BK. A novel reassortant canine H3N1 influenza virus between pandemic H1N1 and canine H3N2 influenza viruses in Korea. J Gen Virol.Mar
93(Pt 3) : 551-4. 2012.
2. Hyungtae Lee, KyoungHwa Jung, Jihyun Park, Gun-woo Ha, Youn-Kyoung Oh, jin-Sik Oh and Young Jyu Chai. Proteomic analysis of the serum from chicken infected by avian influenza virus. J. Bacteriology and virology.
41(3): 195-204.2011.
3. Song DS, An DJ, Moon HJ, Yeom MJ, Jeong HY, Jeong WS, Park SJ, Kim HK, Han SY, Oh JS, Park BK, Kim JK, Pooh, Webster RG, Jung K, Kang BK. Interspecies transmission of the canine influenza H3N2 virus to domestic cats in South Korea, J Gen Viol.
92(Pt 10): 2350-2305. 2011.
4. Song DS, Moon HJ, Jung K, Yeom MJ, Kim HK, Han SY, An DJ, Oh JS, Kim JM, Park BK, Kang BK.. Association between nasal shedding and fever that influenza A (H3N2) induces in dogs.
5. Lee C, Jung K, Oh J, Oh T, Han S, Hwang J, Yeom M, Son D, Kim J, Park B, Moon H, Song D, Kang B. Protective efficacy and immunogenicity of an inactivated avian origin H3N2 canine influenza vaccine in dogs challenged with the virulent virus. Vet Microbiol.
14;143(2-4):184-188.2010.
6. An DJ, Jeoung HY, Jeong W, Chae S, Song DS, Oh JS, Park BK. A serological Survey of Canine Respiratory Coronavirus and Canine Influenza Virus in Korean Dogs. J Vet Med Sci.
7. Choi YJ, Kim HJ, Park JS, Oh MH, Nam HS, Kim YB, Cho BK, Ji MJ, Oh JS.. Evaluation of New Rapid Antigen Test for the Detection of Pandemic Influenza A/H1N1 2009 Virus. J Clin Microbiol.
8. Jeong OM, Kim MC, Kang HM, Ha GW, Oh JS, Yoo JE, Park CH, Kwon JS, Pack MR< Kim HR, Kim YJ, Kwon JH, Lee YJ., Validation of egg yolk antibody based C-ELISA for avian influenza surveillance in breeder duck. Vet Microbiol.
26;144(3-2):287-292.2010
9. Jeong HJ, Song YJ, Lee SW, Lee JB, Park SY, Song CS, Ha GW, Oh JS, Oh YK, Choi IS., Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome Virus. Clin Vaccine Immunol.
7(4):503-512,2010.
10. Chung TH, Oh JS, Lee YS, Kang KS, Jung JW, Youn HY, Hwang CY, Elevated Serum Levels of S100 Calcium Binding Protein A8 (S100A8) Reflect Disease Severity in Canine Atopic Dermatitis. J Vet Med Sci.
11. Lee C, Jung K, Oh J, Oh T, Han S, Hwang J, Yeom M, Son D, Kim J, Park B, Moon H, Song D, Kang B. Protective Efficacy and immunogenicity of an inactivated avian-origin H3N2 canine influenza vaccine in dogs challenged with the virulent virus. Vet Microbiol.
14;143(2-4):184-188.2010
12. Jung K, Lee CS, Kang BK, Park BK, Oh JS, Song DS. Pathology in dogs with experimental canine H3N2 influenza virus infection. Res Vet Sci.
88(3): 523-525.2009.
13. Song DS, Lee YJ, Jeong OM, Kim YJ, Park CH, Yoo JE, Jeon WJ, Kwon JH, Ha GW, Kang BK, Lee CS, Kim HK, Jung BY, Kim JH, Oh JS. Evaluation of a competitive ELISA for antibody detection against avian influenza virus. J Vet Sci.
10(4): 323-329.2009
14. Zhou SH, Cui SJ, Chen CM, Zhang FC, Li J, Zhou S, Oh JS, Development and validation of an immunogold chro-matographic test for on-farm detection of PRRSV.J Virol Methods.
15. Chulseung Lee, Daesub Song, Bokyu Kang, Dongsuk Kang, Jungeun Yoo, Kwonil Jung, Gunsuk Na, Kichang Lee, Bongkyun Park, Jinsik Oh. A serological survey of avian origin canine H3N2 influenza virus in dogs in Korea. Veterinary Microbiology.
16. Daesub Song, Chulseung Lee, Bokyu Kang, Kwonil Jung, Taehoon Oh, Hyekwon Kim, Bongkyun Park,and Jinsik Oh. Experimental infection of Dogs with Avian- Origin Canine Influenza A Virus (H3N2). Emerging Infection Diseases. 
15(1): 56-58. 2009 
17. Cui S, Zhou S, Chen C, Qi T, Zhang C, Oh J. A simple and rapid immunochromatographic strip test for detecting antibody to porcine reproductive and respiratory syndrome virus.  J Virol Methods.
152(1-2): 38-42.2008
18. Daesub Song, Bokyu Kang, Chulseung Lee, Kwonil Jung, Gunwoo Ha, Dongseok Kang, Seongjun Park, Bongkyun Park, Jinsik Oh. Transmission of Avian Influenza Virus (H3N2) to Dogs. Emerging Infectious Diseases.  
14(5): 741-746, 2008.
19. Kim JW, Lee YJ, Han MY, Bae DH, Jung SC, Oh JS, Ha GW, Cho BK  Evaluation of immunochromatographic assay for serodiagnosis of Brucella canis. J Vet Med Sci.
69(11):1103-7. 2007.
20. Park SJ, Song DS, Ha GW, Park BK. Cloning and further sequence analysis of the spike gene of attenuated porcine epidemic diarrhea virus DR13. Virus Genes.
35(1):55-64. 2007.
21. Kang B, Oh J, Lee C, Park BK, Park Y, Hong K, Lee K, Cho B, Song D. Evaluation of a rapid immunodiagnostic test kit for rabies virus.  J. Virol. Methods.
145(1):30-36, 2007.
22. Jung K, Song DS, Kang BK, Oh JS, Park BK. Serologic surveillance of swine H1 and H3 and avian H5 and H9 influenza A virus infections in swine population in Korea. Prev. Vet. Med.
79:294-303, 2007.
23. Song DS, Lee CS, Jung K, Kang BK, Oh JS, Yoon YD, Lee JH, Park BK. Isolation and phylogenetic analysis of H1N1 swine influenza virus isolated in Korea. Virus Res.
125(1):98-103, 2007.
24. Song DS, Oh JS, Kang BK, Yang JS, Moon HJ, Yoo HS, Jang YS, Park BK. Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain. Res. Vet. Sci.
82(1):134-140, 2007.
25. Kang BK, Song DS, Jung KI, Lee CS, Park SJ, Oh JS, An DJ, Yang JS, Moon HJ, Lee SS, Yoon YD, Park BK. Genetic characterization of canine rotavirus isolated from a puppy in korea and experimental reproduction of disease. J. Vet. Diagn. Invest.
19(1):78-83, 2007.
26. Song DS, Kang BK, Oh JS, Ha GW, Yang JS, Moon HJ, Jang YS, Park BK.  Multiplex reverse transcription-PCR for rapid differential detection of porcine pidemic diarrhea virus, transmissible gastroenteritis virus, and porcine group A rotavirus. J. Vet. Diagn. Invest.
18(3):278-281, 2006.
27. Oh JS, Ha GW, Cho YS, Kim MJ, An DJ, Hwang KK, Lim YK, Park BK, Kang B, Song DS. One-step immunochromatography assay kit for detecting antibodies to canine parvovirus. Clin. Vaccine Immunol.
13(4):520-524, 2006.
28. Song DS, Kang BK, Lee SS, Yang JS, Moon HJ, Oh JS, Ha GW, Jang YS, Park BK. Use of an internal control in a quantitative RT-PCR assay for quantitation of porcine epidemic diarrhea virus shedding in pigs. J. Virol. Methods.
33(1):27-33, 2006.
29. Oh JS, Song DS, Yang JS, Song JY, Moon HJ, Kim TY, Park BK. Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection. J. Vet. Sci.
6(4):349-352, 2005.
30. DaeSub Song, JinSik Oh, BoKyu Kang, JeongSun Yang, JuYoung Song, HyoungJoon Moon, TaeYung Kim, HanSang Yoo, YongSuk Jang, BongKyun Park. Fecal shedding of a highly cell-culture-adapted porcine epidemic diarrhea virus after oral inoculation pigs. Journal of Swine Health and Production.
13(5):269-272, 2005.
31. Oh JS, Song DS, Yang JS, Song JY, Yoo HS, Jang YS, Park BK. Effect of soluble porcine aminopeptidase N on antibody production against porcine epidemic diarrhea virus. J. Vet. Sci.
5(4):353-357, 2004.
32. Oh JS, Song DS, Park BK. Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes. J. Vet. Sci.
4(3):269-275, 2003.
33. Song DS, Yang JS, Oh JS, Han JH, Park BK. Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF3. Vaccine.
21:1833-1742, 2003.
34. Song DS, Lee JY, Oh JS, Lyoo KS, Yoon KJ, Park YH, Park BK. Isolation of H3N2 swine influenza virus in South Korea. J. Vet. Diagn. Invest.
15(1):30-34, 2003.


특허 현황


특허 제 10-0574559호


신속 면역크로마토그래피법에 의한 개 파보 항체 진단 키트 및 그 제조방법


특허 제 10-0593286호


신속 면역크로마토그래피법에 의한 개 디스템퍼 항체 진단 키트 그 제조방법


특허 제 10-0762825 호


신속 면역 크로마티그라피법에 의한 H5형 고병원성 및 일반 조류 인플루엔자 바이러스 항원 진단 스트립 및 그 제조 방법

특허 제 10-080545호


신규한 개 인플루엔자 바이러스 및 이의 백신



특허 제 10-0954992호


경쟁적 효소면역측정법을 이용한 조류 인플루엔자 바이러스 항체의 진단키트 및 검출방법